Measurement of complementary interaction, e.g. in immunoassays - comprising measurement of fluorescence or redox (current) strength with double measurements and comparisons of at least two measuring zones

摘要

Claimed is (A) a sensitive method for immunoassays or assays based on complementary interaction by (a) quick and repetitive measurements of electric current or fluorescence, and (b) double measurements either in at least two measuring zones or by internal calibration of the system using stable redox or IR-fluorescence-labelled analytes followed by comparison of the two measurements obtd.. Also claimed is an appts. for performing (A) wherein the analytes recognising complementary mols. (antibodies (ABs), receptors, DNA-fragments) are immobilised at special surfaces. USE/ADVANTAGE - The new method is used in the detection of binding pairs such as in immunoassays. This method is simpler, faster, more correct, cheaper and more sensitive than known methods such as ELISAs RIAs or EIAs.