摘要 |
<p>Disclosed are methods for producing substantially pure EG III cellulase from Trichoderma spp. In particular the methods of the present invention comprise separation of the EG III cellulase component from an aqueous mixture of enzymes by the addition of polyethylene glycol to the aqueous mixture to create a two-phase system and separating the EG III-rich phase. The present invention is also directed in part to methods for preparing substantially pure EG III cellulase component by fractionation or by a combination of the above two methods.</p> |