| 摘要 |
<p>Fluorogenic chelators for Eu?3+ and Tb3+¿ are provided, they form highly fluorescent complexes with Eu?3+ and Tb3+¿. In all cases, the fluorescence observed was lanthanide-specific, long-lived and it was monitored by microsecond time-resolved fluorometry. The fluorogenic chelators could be quantified, in the presence of excess lanthanide, at levels < 10-8 mol/L. Fluorogenic chelators can form ternary complexes with Eu?3+ and Tb3+¿, in the presence of EDTA. The structures of the identified chelators is such that enzyme substrates can be used for enzyme-labelled time-resolved fluorometric immunoassays.</p> |
