摘要 |
<p>The clostripain obtained from the culture filtrate of Clostridium histolyticum DSM 627 is employed for the coupling of arginine with amino-terminal protection or of peptides with amino-terminal protection and with carboxy-terminal arginine (acyl donor) with an amino acid or a peptide with amino-donor function. The enzyme can be obtained in high purity by alcohol precipitation and subsequent adsorption of the enzyme which has been obtained from the culture filtrate after alcohol precipitation onto a reactive dye, and be used for the coupling. The specific activity of clostripain is at least 80 U/mg. Clostripain catalyses not only the abovementioned coupling of proteinogenous amino acids but also the coupling of a proteinogenous with a non-proteinogenous amino acid.</p> |