| 摘要 |
An immortalized cell line that produces antigen-specific human monoclonal antibody is described as well as methods for making this cell line. The cell line is derived from fusion of a human chromosome containing heterohybrid fusion partner with peripheral blood lymphocytes of a first seropositive donor. A mixed lymphocyte response is generated by incubating the peripheral blood lymphocytes in the presence of allogeneic lymphocytes. When the selected antigen is included in the incubation mixture, the peripheral blood lymphocytes of the first seropositive donor form secondary lymphoblast cells which are fused to the heterohybrid fusion partner. The resulting secondary immune response generates monoclonal antibodies of high affinity. A cell line which produces monoclonal human anti-IgE antibody is disclosed. |
