| 摘要 |
PURPOSE:To industrially and advantageously obtain a new physiologically active substance expected to have utility as an anti-cancer medicine, having inhibitory activity against DNA polymerase alpha by culturing a haploid ameba of Physarum polycephalum of true Myxomycetes in a culture. CONSTITUTION:A haploid ameba of Physarum polycephalum MCI-2,526 of true Myxomycetes is inoculated into a liquid medium, cultured at 24 deg.C for one day at a dark place, 2-3 drops of the culture solution is dripped on an agar medium, spread to the whole face, further cultured at 24 deg.C for two days at a dark place, Aerobacter aerogenes MCI-2,517 as a feed of the ameba of Myxomycetes is inoculated to the agar medium and the haploid ameba is cultured at 24 deg.C for four to five days at a dark place. The haploid ameba is collected from the agar medium, subjected to an ultrasonic grinder and centrifuged to collect a supernatant, which is extracted with a chloroform/methanol solvent and the extract is purified by chromatography to give the objective new physiologically active substance of the formula. |
