| 摘要 |
<p>Disclosed is a method for the treatment of a patient infected with an intracellular pathogen involving administering to the patient a sub-sample of the patient's own PBMCs enriched for pathogen-specific cytotoxic T lymphocytes. Such a CTL sub-sample is prepared by (i) isolating a cell from the mammal; (ii) expressing in the cell a nucleic acid which encodes a pathogen-specific polypeptide, whereby the polypeptide is processed by the cell and an antigenic portion of the polypeptide is presented on the cell surface; and (iii) contacting this antigen-presenting cell with a sample of the mammal's peripheral blood mononuclear cells (PBMCs) to stimulate the production of the enriched sub-sample of CTLs which recognize and which are capable of lysing pathogen-infected cells of the mammal.</p> |
