发明名称 |
ELECTROCHEMICAL TREATMENT OF NUCLEIC ACID CONTAINING SOLUTIONS |
摘要 |
A process is described for denaturing native double-stranded nucleic acid material into its individual strands in an electrochemical cell. The process is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process employs a promoter which is an inorganic multivalent cation such as magnesium ions to speed denaturation. The process may be used in the detection of nucleic acid by hybridrising with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction. |
申请公布号 |
WO9315224(A1) |
申请公布日期 |
1993.08.05 |
申请号 |
WO1993GB00147 |
申请日期 |
1993.01.22 |
申请人 |
SCIENTIFIC GENERICS LIMITED |
发明人 |
STANLEY, CHRISTOPHER, JOHN;ARCHER, PATRICIA, LYNN |
分类号 |
C12N15/09;C12N15/10;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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