| 摘要 |
The invention relates to an instrument for measuring a biochemical reaction by measuring the deflection of light in a planar optical waveguide. This deflection results from the biochemical reaction taking place and is caused by the ligands to be detected growing with a nonuniform layer thickness on the waveguide surface. This can be caused by the receptor layer being applied in such a way to the waveguide surface that a chemical gradient results, the gradient being perpendicular to the propagation direction of the light passing through the waveguide, while said receptor layer is uniform in optical terms (layer thickness, refraction index profile). An alternative possibility, however, is for the receptor layer to be uniform both in optical and chemical terms but for the ligands to be supplied nonuniformly (flow, diffusion). In both cases the result is growth of a nonuniform ligand layer, resulting in a deflection of the light being propagated through the waveguide. The major advantage of this deflection sensor is that it is insensitive to changes in refractive index in the liquid in which the instrument is placed.
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