Method for the selection of at least one mutations' screen, its application to a rapid identification of polymorphic systems' alleles and apparatus for carrying it out.

摘要

Methods for the selection of at least one mutation screen from a set of allelic sequences of a polymorphic gene and for the rapid identification of alleles of polymorphic genes, nucleotide probes obtained from the said mutation screens, especially consisting of a data bank and device for implementing the said methods. The method for the identification of alleles comprises: (a) the selection of all or part of a known consensus sequence of the said polymorphic gene; (b) the creation of a template for the mutations of the corresponding sequences of known alleles; (c) the identification of the indiscernible sequences by comparison in pairs (alleles having the same mutation profile in the sequence selected in (a)) and the exclusion of one of the members of the said pairs; (e) the identification and the enumeration of the obligatory mutations or the so-called allele marker mutations, that is to say those which are required or sufficient for distinguishing two alleles which are otherwise identical (set O of obligatory mutations); and (f) the production of the said minimum mutation screen(s) comprising at least the obligatory mutations of step (e); and then (g) the selection, among the screens selected in step (f) of the said method for the selection of mutation screens, of the mutation screen which is most suitable for the preparation of oligonucleotide probes suitable for use in the differentiation of all the alleles; (h) an appropriate hybridisation of an allele X to be identified with the oligonucleotide probes selected from the mutation screen(s) established in steps (a) to (g); and (i) identification of the allele X by detecting the said hybrid(s) optionally formed in step (h).