| 摘要 |
Tissue plasminogen activator (t-PA) variants with substitution of leucine fa r phenylalanine, histidine for arginine, serine for isoleucine and threonine for lysine at amino acid positions (274, 275, 2 76 and 277) respectively, of wild-type human t-PA are prepared. DNA sequences can be prepared that encode such variants, as well a s expression vectors incorporating the DNA se- quences, and host cells transformed with the expression vectors. The variant s exhibit improved fibrin-specificity and can be used in a pharmaceutical preparation to treat a vascular disease or condition or to prevent fibrin deposition or adhesion formation or reformation in mammals.
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