发明名称 |
METHOD FOR REFOLDING IGF-1 TO ACTIVE CONFORMATION |
摘要 |
2122837 9311240 PCTABS00022 A method for refolding insoluble, improperly folded IGF-I is provided, wherein the IGF-I, precipitated from prokaryotic host cells, is concurrently solubilized, unfolded, and refolded into a biologically active conformation in a single buffer. The buffer contains reducing agent and chaotropic agent to solubilize the IGF-I at concentrations sufficiently low to allow solubilization and refolding to occur. Also provided is a triple-protease deficient E. coli host suitable for use in the process.
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申请公布号 |
CA2122837(A1) |
申请公布日期 |
1993.06.10 |
申请号 |
CA19922122837 |
申请日期 |
1992.12.04 |
申请人 |
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发明人 |
CHANG, JUDY Y.;MCFARLAND, NANCY C.;SWARTZ, JAMES R. |
分类号 |
C07K1/02;C07K1/113;C07K14/575;C07K14/65;C12N1/21;C12N15/00;C12N15/16;C12P21/02;C12R1/19;(IPC1-7):C12N15/16;C07K1/107 |
主分类号 |
C07K1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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