| 摘要 |
2123906 9310250 PCTABS00022 The present invention relates to a protein or protein complex which stimulates activated DNA transcription in eukaryotic cells, genes encoding the protein or proteins, and uses therefor. The protein or protein complex, referred to herein as a transcriptional adaptor, is associated with two other factors, each of which, in turn, binds to a region of a eukaryotic promoter. The first of these factors, referred to herein as an activator, binds to a region of the promoter DNA sequence termed the UAS binding site or enhancer. The second factor or factors, referred to herein as a general transcription factor (GTF), binds to second region of the promoter DNA sequence, termed the TATA box, which is located downstream from the UAS binding site. The transcriptional adaptor specifically binds to an acidic activation domain (i.e., one which is highly enriched in acidic amino acids, such as aspartic acid and glutamic acid) on the activator protein which, in addition, includes a DNA binding domain.
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