摘要 |
This invention relates to a novel method for assaying 1,5-anhydroglucitol, which is expected to serve as a marker for diabetes, and a kit therefor. More particularly, it relates to: (1) a method for assaying 1,5-anhydroglucitol, which comprises selectively removing sugars from a specimen containing 1,5-anhydroglucitol; allowing pyranose oxidase or L-sorbose oxidase to act on 1,5-anhydroglucitol contained in the sample thus obtained in the presence of oxygen; and determining 1,5-anhydroglucitol from the amount of the hydrogen peroxide thus formed; and (2) a reagent kit for assaying 1,5-anhydroglucitol which comprises an agent for removing sugars, a reagent for detecting hydrogen peroxide and an enzyme for oxidizing 1,5-anhydroglucitol. s |
申请人 |
NIPPON KAYAKU K.K., TOKIO/TOKYO, JP |
发明人 |
NAKAMURA, TSUNEO, OOMIYA-SHI SAITAMA-KEN, JP;AKANUMA, HIROSHI, YOKOHAMA.-SHI KANAGAWA-KEN, JP;YABUUCHI, MASAHIKO, OOMIYA-SHI SAITAMA-KEN, JP;KATO, KAZUO, URAWA-SHI SAITAMA-KEN, JP;MASUDA, MINORU, AGEO-SHI SAITAMA-KEN, JP |