| 摘要 |
PURPOSE:To improve accuracy of a sample quantity measurement by pressing an inactive support from the outside against the hollow hole part provided on the wall of a vessel in which flow passages to introduce and discharge various sorts of solution, a stirring means, and a detecting electrode are provided, at measuring the sample quantity to be detected. CONSTITUTION:A sample is caught and marked by an inactive support to which a first antibody or antigen to combine with the sample in sample solution by immunity reaction is immobilized, and by an enzyme-marked second antibody or antigen, next, enzyme activity of the inactive support, and an enzyme reacted quantity is detected by using an electrode so as to measure the sample quantity in the sample, solution. This vessel 1 is provided with a polar type acid electrode 2, mounted thereon and an antibody fixing silk fibroin membrane 4 is pressingly mounted on the circular hollow hole part on the opposite face by means of a supporting plate 5. The sample solution and marked antibody solution are mixed at a fixed ratio, held in a pouring part for a fixed time, then fed to the vessel 1 through an introducing pipe 9, but prior to it bubbles in the vessel are removed and surplus solution is drained through pipings 10, 11. Measuring operation is automatically performed in a short time. |
