VERFAHREN UND REAGENZ ZUR BESTIMMUNG VON ALPHA-AMYLASE.

摘要

Determination of alpha-amylade (AA) comprises cleavage of a substrate in presence of at least one auxiliary enzyme, then measuring the cleavage products. The new feature is that reaction is carried out in presence of a monoclonal antibody (MAB) against AA. Also new are reagents for this process. Pref. the substrate is a D-maltooligoside of 2-10, esp. 4-8, glucose units, opt. substd. by a chromophore (esp. nitrophenyl) and/or having at least one ethylidene protecting gp. MAb are esp. derived from the hybridoma clones ECACC 86020601 and ECACC 86020602.