发明名称 |
PROMOTER LIGATION ACTIVATED TRANSCRIPTION AMPLIFICATION OF NUCLEIC ACID SEQUENCES |
摘要 |
This invention discloses a scheme for producing nucleic acid end products that are functionally or exactly identical to the starting products, thereby resulting in exponential amplification of a desired nucleic acid sequence. Specifically, sequences are cycled between RNA and DNA forms using the following basic steps: (1) a T7 RNA polymerase promoter is ligated onto a single-stranded DNA template; (2) T7 RNA polymerase makes many copies of RNA: (3) a complementary DNA is made from the RNA by extension of a primer by reverse transcriptase; and (4) the RNA template is removed by ribonuclease H. This amplification method is useful for purposes such as genetic research and diagnostic assays.
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申请公布号 |
US5194370(A) |
申请公布日期 |
1993.03.16 |
申请号 |
US19900524306 |
申请日期 |
1990.05.16 |
申请人 |
LIFE TECHNOLOGIES, INC. |
发明人 |
BERNINGER, MARK S.;SCHUSTER, DAVID M.;RASHTCHIAN, AYOUB |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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