发明名称 |
IMPROVED METHOD OF AMPLIFYING TARGET NUCLEIC ACIDS APPLICABLE TO BOTH POLYMERASE AND LIGASE CHAIN REACTIONS |
摘要 |
The present invention involves methods of improving LCR and PCR amplification schemes by modifying at least one probe/primer end so that the probability of the probe/primer contributing to spurious signal development is greatly reduced. Only after specific hybridization of the modified probe/primer with true target, are the modified ends "corrected" in a target dependent fashion to allow participation of the probe/primer in the enzymatic assembly reaction. Specific modifications depend on whether the assembly is done by ligation (as in LCR) or by extension/elongation (as in PCR). <IMAGE> |
申请公布号 |
AU635105(B2) |
申请公布日期 |
1993.03.11 |
申请号 |
AU19910070007 |
申请日期 |
1991.01.25 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
KEITH C. BACKMAN;SHELIA B. BOND;JOHN J. CARRINO;THOMAS G. LAFFLER |
分类号 |
C12Q1/25;C07H21/00;C12N15/09;C12Q1/48;C12Q1/68 |
主分类号 |
C12Q1/25 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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