| 摘要 |
PURPOSE:To highly sensitively and rapidly detect a substance to be detected moving in a capillary irrespective of its position. CONSTITUTION:An electrophoretic apparatus comprises a capillary 2, a positive pole buffer reservoir 3, a negative pole buffer reservoir 4, a light source 5, an optical detector 6 and a power source 7. Applied light 8 from the light source 5 is coaxial with a part of the capillary 2 and the applied light 8 passes through the capillary 2. The optical detector 6 is placed in parallel with a part of the capillary 2 which is coaxial with the applied light 8 from the light source 5. A substance 9 to be detected is separated while it is moving in the capillary 2 by applying voltage to both ends of the capillary 2. The substance 9 to be detected is excited by the applied light 8 and generates fluorescent light 10. The fluorescent light 10 is detected by the optical detector 6. |
