| 摘要 |
PURPOSE:To efficiently and economically obtain high-viscous cultured product such as beta-glucan without exerting adverse influence to hypha by aerobically subjecting a filamentous fungi belonging to the genus Macrophomopsis to liquid culture while stirring the cultured product with a paddle type agitator. CONSTITUTION:A filamentous fungi strain KAB 55 (FERM P-9366) of the genus Macrophomosis, etc., is cultured in a medium for 3 day and planted in a jar fermentor to which a paddle type agitator is fitted and aerobically cultured at 25 deg.C for 4 day to produce highly viscous cultured product and then the cultured product is centrifuged to remove a microbial fungal cell and an organic precipitating agent such as ethanol is added to the supernatant. Thereby the product is subjected to precipitation separation to provide the objective cultured product containing high-viscous beta-glucan, wherein all D-goucopyranosyl residues of the main chain have beta-1,3 bond and branched at C-6 position and D- glucopyranosyl residues of the side chain have beta-1,6 bond in a ratio of 1 based on 4 D-glucopyranosyl residues. |
