FREMGANGSMAADE TIL FREMSTILLING AF MIKROBIELLE PROTEINER, SOM ER OPNAAET UD FRA CHLAMYDIA TRACHOMATIS, TIL IMMUNOBESTEMMELSE

摘要

In a method of specimen treatment preparatory to conducting an immunoassay, a microbial protein is solubilized by a detergent at elevated temperature and in the presence of an alkali or alkaline earth metal ion. At elevated temperatures, the detergent is soluble. However, at lower temperatures, the presence of the metal ion renders the detergent insoluble so that it is prevented from interacting in the immunoassay procedure. A specific application is in the solubilization of the principal outer membrane protein of Chlamydia trachomatis.