| 摘要 |
<p>A method of detecting gene deletions includes the steps of obtaining a genomic DNA sample possibly including a gene deletion, the gene having two ends normally flanked by two homologous regions having substantially identical portions, the gene deletion being in the form of a fusion fragment of the two homologous regions. The genomic DNA is amplified using a set of primers capable of amplifying both the fusion fragment and the two normal homologous regions having the gene in-between. The amplified fusion fragments are characterized alone indicating a homozygous deletion, a combination of fusion fragments and the two homologous regions and the gene in-between indicating a heterozygote deletion, or the two homologous regions and the gene alone indicating a homozygous nondeletion. Novel primers for use in the inventive method are also disclosed.</p> |
