发明名称 |
MOLECULAR DETECTION OF GENE DELETIONS |
摘要 |
A method of detecting gene deletions includes the steps of obtaining a genomic DNA sample possibly including a gene deletion, the gene having two ends normally flanked by two homologous regions having substantially identical portions, the gene deletion being in the form of a fusion fragment of the two homologous regions. The genomic DNA is amplified using a set of primers capable of amplifying both the fusion fragment and the two normal homologous regions having the gene in-between. The amplified fusion fragments are characterized alone indicating a homozygous deletion, a combination of fusion fragments and the two homologous regions and the gene in-between indicating a heterozygote deletion, or the two homologous regions and the gene alone indicating a homozygous nondeletion. Novel primers for use in the inventive method are also disclosed. |
申请公布号 |
WO9300445(A1) |
申请公布日期 |
1993.01.07 |
申请号 |
WO1992US04764 |
申请日期 |
1992.06.04 |
申请人 |
VANDERBILT UNIVERSITY |
发明人 |
PHILLIPS, JOHN, A., III;VNENCAK-JONES, CINDY, L. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|