发明名称 |
METHODS FOR PRODUCING MEMBERS OF SPECIFIC BINDING PAIRS |
摘要 |
The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described. |
申请公布号 |
AU1693892(A) |
申请公布日期 |
1992.12.30 |
申请号 |
AU19920016938 |
申请日期 |
1992.05.15 |
申请人 |
CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED |
发明人 |
GREGORY PAUL WINTER;KEVIN STUART JOHNSON;ANDREW DAVID GRIFFITHS;ANDREW JOHN HAMMOND SMITH |
分类号 |
A61K39/395;C07K1/22;C07K16/00;C07K16/18;C07K16/22;C07K16/24;C07K16/28;C07K16/30;C07K16/34;C07K16/40;C07K16/42;C07K16/44;C07K16/46;C07K19/00;C12N15/00;C12N15/09;C12N15/10;C12N15/13;C12N15/62;C12N15/73;C12P21/02;C12P21/08;C12R1/19;C40B40/02;G01N33/53;G01N33/531;G01N33/68 |
主分类号 |
A61K39/395 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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