| 摘要 |
A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10+/-0.5; (ii) an optimum temperature for activity of about 45 DEG to 55 DEG C.; (iii) an optimum pH for stability of about 7.0+/-0.5; (iv) a molecular weight as measured by SDS-PAGE of about 3.0x104; and (v) chemical stability for at least a 60 day mean half-life in the presence of a 10 percent solution of polyoxyethylene (23) lauryl ether in 25 millimolar aqueous calcium chloride. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase.
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