PROCESS AND DEVICE FOR SPECIFIC BINDING ASSAY

摘要

An assay process and a device useful for the practice of the assay process, effective for high sensitivity semiquantitative assay, in which assay results can be obtained by simply loading a sample as it is and any desired detection sensitivity can be selected at will without causing decrease (or increase) in the maximum intensity of signals. Particularly, this invention provides a process for specific binding assay in which a substance in a test sample to be assayed is detected qualitatively or quantitatively making use of specific binding reaction, which comprises including a specified substance in an assay system and, by the effect of the presence of the specified substance, decreasing (or increasing) amounts of a labeled material to be measured as an index of the amount of the substance to be assayed. It also provides a chromatography type specific binding assay device which comprises a means for loading a test sample, a means for locating specific binding substances, a means for locating a detecting element and a means for absorbing liquid materials in that order, wherein a specific binding substance and a labeled specific binding substance are included in the means for locating specific binding substances and a detecting element on which a specific binding substance is immobilized is included in the means for locating a detecting element.