| 摘要 |
PURPOSE:To obtain the subject new peptide capable of inhibiting angiotensinase having vasopressor activity and useful for a health food, a medical drug, etc., for prevention of hypertension by decomposing royal jelly using a protease. CONSTITUTION:Royal jelly protein is added to a phosphate buffer solution (pH2) and a protease such as pepsin is further added thereto. After decomposition of the protein by incubation at 37 deg.C for 24hr, the enzyme is inactivated by heat treatment in boiling water for 10min. The treated solution is then allowed to cool and subsequently centrifuged to remove the insoluble materials. The resultant supernatant is fractionated by a gel filtration and active fractions are passed through an adsorptive resin column. The adsorbed fraction is subjected to gradient elusion by using ammonium formate and its active fraction is then further purified by high performance liquid chromatography, etc., thus obtaining the objective new peptide having an amino acid sequence of the formula and having an angiotensinase inhibitory effect. |
