发明名称 |
CDNA CLONE ENCODING A HUMAN NOREPINEPHRINE TRANSPORTER |
摘要 |
2106190 9217568 PCTABS00016 A cDNA encoding human norepinephrine transporter isolated from human neuroblastoma cells is disclosed. Determination of the nucleotide sequence thereof permitted a putative amino-acid sequence of the transporter protein to be determined. Mammalian cells transfected with the cDNA produced an mRNA species similar in size to that produced by norepinephrine-producing neurons. Such transfected cells also became capable of norepinephrine uptake. The uptake was inhibitable by various uptake-inhibiting drugs, including cocaine, to a degree similar to the effect of such drugs on noradrenergic neurons. The cloned cDNA makes possible well-controlled studies of transporter function in non-neuronal cells without the obfuscating influence of other transporters in the same cells. Such studies include the relative effects of various drugs such as antidepressants.
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申请公布号 |
CA2106190(A1) |
申请公布日期 |
1992.09.29 |
申请号 |
CA19922106190 |
申请日期 |
1992.02.20 |
申请人 |
STATE OF OREGON ACTING BY AND THROUGH THE STATE BOARD OF HIGHER EDUCATION ON BEHALF OF OREGON HEALTH SCIENCES UNIVERSITY (THE) |
发明人 |
AMARA, SUSAN G.;PACHOLCZYK, TADEUSZ;BLAKELY, RANDY D. |
分类号 |
C12N15/09;C07K;C07K14/435;C07K14/705;C12N5/00;C12N5/10;C12N15/12;C12N15/79;C12P21/02;C12R1/91;(IPC1-7):C12N15/12;C07H21/00;C12N1/21;C07K14/47 |
主分类号 |
C12N15/09 |
代理机构 |
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