| 摘要 |
The method of electrophoresis uses for separation of charged species their different mobilities in an electric field. The pro- cess is usually carried out by forcing the molecules to migrate through an aqueous gel. The gels are run in essentially two types of electrophoretic units: vertical and horizontal ones. It was found that in the standard submerged electrophoresis units the resolu- tion of DNA in the poly(NAT) gels was never so good as in the vertical system. It was observed that by looking at the gel from above under an angle declined from the vertical, the viewed DNA bands in the gel were much sharper than they were pictured on the photograph taken by a camera positioned more or less vertically above to gel. On basis of this observation it was assumed that the diffuseness of bands seen on the photograph did not originate from a real diffuseness of bands in the gel, but rather from the vertical position of the camera and bending of the bands against the vertical axis. If the bands in the gel could be made essential- ly vertical, then the resolution taken by the usually positioned camera would be qualitatively better and besides that independent of the sample volume. It is the main goal to prevent such bending of bands in gels during electrophoresis.
|
