A DNA segment contains a GAL1 promoter of Saccharomyces cerevisae linked to a gene other than the galoctokinase gene, for directing the expression of the gene within a yeast cell. A GAL1 promoter portion of Saccharomyces cerevisae is linked to a foreign DNA segment for use in expressing a desired protein. Yeast cells containing the GAL1 promoter linked to a foreign DNA segment are grown in a medium containing glucose, wherein the yeast cells metabolize the glucose and are permitted to express a polypeptide when galactose is present in the medium.
申请公布号
US5139936(A)
申请公布日期
1992.08.18
申请号
US19880283812
申请日期
1988.12.13
申请人
COLLABORATIVE RESEARCH, INC.
发明人
BOTSTEIN, DAVID;DAVIS, RONALD W.;FINK, GERALD R.;TAUNTON-RIGBY, ALISON;KNOWLTON, ROBERT G.;MAO, JEN-I;MOIR, DONALD T.;GOFF, CHRISTOPHER G.