| 摘要 |
<p>A capillary zone electrophoresis (CZE) chemical analysis system ( 100) provides for rapid, non-turbulent, post-separation diffusional mixing of sample effluent with a fluorogenic labeling reagent (130), permitting sensitive detection of well-defined sample component zones. A power supply (102) and opposing electrodes (120 and 124) establish a field which induces charge-related differential electrophoretic migration to define component zones. A flared input (253) of a mixing capillary (114) defines an annular gap (252) between the separation capillary (110) and the mixing capillary. The effluent of the separation capillary is mixed with the labeling reagent (130), which is introduced through the annular gap. Thus, fluorescence labeling is effective with minimum zone broadening. This system combines the high resolving power of CZE separation with the sensitivity of labeled fluorescence detection to attain an improved system for analyzing biological samples. <IMAGE></p> |
