METHOD OF IMMOBILIZING AND CROSSLINKING PROTEINS AND OTHER MOLECULES AND USES THEREOF

摘要

X8180A The invention provides a method for immobilizing and purifying proteins. The invention further provides a method for the formation of a kinetically inert complex between a transition metal ion and a Biologically Active Molecule or reporter group which possesses a metal binding site to form a kinetically inert complex between the CP-protein and the bound metal ion. This kinetically inert ¢immobilized metal/CP-protein! complex provides a component of an assay system useful for studying the interaction of any of a variety of ligands with the immobilized CP-protein. The invention further provides a method of purifying IPs or receptors on a Solid Support. Immobilization of IPs or other Biologically Active Molecules utilizing the methodology of the instant invention enables one to orient these molecules so as to maximize exposure of the antigen or ligand binding site in an affinity chromatography system. Furthermore the invention provides a method of forming heterodimeric, homodimeric or multimeric complexes by crosslinking two or more Biologically Active Molecules or reporter groups with metal binding sites.