| 摘要 |
<p>Pleiotropic pro-inflammatory cytokines, such as TNF and IL-1, induce expression of a protein molecule, termed TSG-14, in connective tissue cells. The TSG-14 protein and functional derivatives thereof, DNA coding therefor, expression vehicles, such as a plasmid, and host cells transformed or transfected with the DNA molecule, and methods for producing the protein and the DNA are provided. Antibodies specific for the TSG-14 protein are disclosed, as is a method for detecting the presence of TSG-14 protein in a biological sample, using the antibody or another molecule capable of binding to TSG-14 such as hyaluronic acid. A method for detecting the presence of nucleic acid encoding a normal or mutant TSG-14 protein, a method for measuring induction of expression of TSG-14 in a cell using either nucleic acid hybridization or immunoassay, a method for identifying a compound capable of inducing the expression of TSG-14 in a cell, and a method for measuring the ability of a cell to respond to TNF are also provided.</p> |
