IN-SITU HYBRIDIZATION PROBES FOR IDENTIFICATION AND BANDING OF SPECIFIC HUMAN CHROMOSOMES AND REGIONS

摘要

The invention relates to novel primer sets useful in preparing DNA probes specific for any chromosome or part of a chromosome, particularly human chromosomes. The DNA probes so produced may be used to paint individual chromosomes or portions of chromosomes in metaphase cell spreads and in interphase nuclei. When used to paint chromosomes in metaphase spreads, R-bands are readily detectable. The method is sensitive and has been shown to paint R-bands on chromosomes pieces having as few as several hundred kilobases.