发明名称 |
Method of immobilizing single-stranded DNA on carrier at terminal. |
摘要 |
<p>A method of immobilizing a single-stranded DNA on a carrier at the terminal, which comprises binding a double-stranded DNA comprising the single-stranded DNA and its complementary strand to the carrier only at the terminal of the single-stranded DNA and subsequently detaching the complementary strand from the single-stranded DNA by means of denaturing treatment, provided that the single-stranded DNA contains one or more extra nucleotide molecule(s) at the terminal when compared with the complementary strand or the terminal nucleotide molecule of the single-stranded DNA has been chemically modified so that the single-stranded DNA can bind to the carrier via the extra nucleotide molecule(s) or the chemically-modified terminal nucleotide molecule.</p> |
申请公布号 |
EP0487104(A1) |
申请公布日期 |
1992.05.27 |
申请号 |
EP19910119961 |
申请日期 |
1991.11.22 |
申请人 |
SUMITOMO ELECTRIC INDUSTRIES, LTD |
发明人 |
KISHIMOTO, TOSHIHIKO;NIWA, SHIN-ICHIRO;NAKABAYASHI, MAKOTO |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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