| 摘要 |
A method for the preparation of a 3' end functionalized polynucleotide is disclosed. An aminefunctionalized solid phase support is treated sequentially with an anhydride, then with an 1hydroxylamine. A polynucleotide is chemically synthesized on the treated support and is subsequently cleaved therefrom by hydrolysis of the amide bonds. A polynucleotide having a 3' free primary amine is recovered for use in hybridization assays and other uses. |
