| 摘要 |
Compounds of the formula DNB-TZ(P2) (P3) X, wherein TZ is a tetrazolium ring, DNB is 5-(2,4-dinitrophenyl), X is halide and P2 and P3 are each independently halophenyl, nitrophenyl or phenyl. The compound INDT 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-dinitrophenyl) tetrazolium bromide exhibits facile reduction to insoluble chromophoric formazan compared to the chromagen INT. INDT differs structurally from INT 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride by having 2,4-dinitrophenyl instead of phenyl at the 5-position on the tetrazolium ring. The compounds, including INDT, are useful as chromagens for histological staining, as well as in enzyme-amplified staining as a part of immunological or hybridization assays.
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