MEASUREMENT OF COLOR REACTIONS BY MONITORING A CHANGE OF FLUORESCENCE

摘要

The present invention relates to a process to determine the concentration of any substance in a colorimetric, turbidimetric or nephelometric reaction using a fluorometric detector to measure fluorescence intensity. In particular, a change in color can be monitored by observing the measurement of fluorescence intensity of a fluorophore in an inert matrix. The absorption spectrum of the chromophore may overlap the excitation and/or emission spectrum of the fluorophore, thereby allowing the change in fluoroscence to be related to the intensity of color in the reaction and thus related to the quantity of the substance of interest.