| 摘要 |
LIQUID CHROMATOGRAPHIC SYSTEM To supply a programmed gradient to a highpressure pump for introduction into a column, a mixer, degasser and accumulator communicates with the high-pressure pump to supply fluid thereto, having a volume no more than 150 times the chamber volume of the high-pressure pump. The mixer, degasser and accumulator includes temperature sensors which sense when the mixer, degasser and accumulator has been emptied to a level where it cannot respond to a demand for fluid from said highpressure pump and provides a signal to a lowpressure pump which responds by cycling to again fill the mixer, degasser and accumulator. Upon receiving a demand signal, the low-pressure pump fills by drawing fluid from a plurality of fluid sources to compose the gradient being used at that time, with the pump slowing during valve opening and losing so as to avoid cavitation. |
