| 摘要 |
PURPOSE:To efficiently obtain the subject plasmid by containing a promoter region, etc., of alpha-galactosidase gene extracted from Saccharomyces oleaginosus (SOL). CONSTITUTION:A fungus derived from cultivation of SOL IFO 1998 strain is extracted to obtain a chromosome DNA and said DNA is subjected to restrictive enzyme treatment with BamHI or Sau3A to obtain partially decomposed DNA. Next, said DNA is decomposed to fractions to obtain a plasmid (A) containing a promoter region, a secreted sequence region and a structural gene region of alpha-galactosidase gene. Next, the component A is inserted into a BamHI position of a tetracycline-resistant gene of a plasmid vector YEp13 (B) as a shuttle vector to obtain a plasmid vector (C). Then, a transformant (e.g. Saccharomyces cerevisiae) obtained by inserting the component C is cultured to digest raffinose, etc., as a non-fermenting saccharide. |
