| 摘要 |
2094821 9207944 PCTABS00013 This application concerns vaccinia vectors and it is based upon the discovery of three unobvious gene sequences in the viral genome. One or more of the gene sequences may be inactivated, or part of all of one or more of these gene sequences may be deleted from the viral genome to allow (i) greater attenuation of the virus and/or (ii) enhancement of immunogenicity of recombinant vaccinia virus; and/or (iii) further gene sequence insertion sites, so that more foreign DNA may be incorporated in the virus. Where however, the gene sequences are essential for viral replication, viral attenuation can still be effected by altering the gene product (e.g. by manipulation at gene level ), such that a protein function effecting pathogenicity is adversely affected, whilst keeping the protein functional for virus replication.
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