A PROCESS FOR THE GENE MANIPULATION OF PLANT CELLS, RECOMBINANT PLASMIDS, RECOMBINANT BACTERIA, PLANTS

摘要

A process for the gene manipulation of cells of plants which, in essence, cannot be transformed by Agrobacterium tumefaciens with integration by T-DNA, which comprises introducing foreign DNA into the plant cells by infecting the plant cells with one or more recombinant Agrobacterium tumefaciens strains which contain foreign DNA to be transferred to the plant cells between the left- and right-hand ends of T-DNA necessary for such a transfer and are capable of transferring this foreign DNA to the plant cells, the foreign DNA located between the left- and right-hand ends of T-DNA comprising: (A) a transposase gene located in an expression cassette active in the plant cells but not beteween the left- and right- hand transposon ends necessary for integration in the DNA of the plant cells, and (B) a recombinant transposon comprising the left- and right- hand transposon ends necessary for integration in the DNA of the plant cells with an intermediate DNA fragment to be integrated in the DNA of the plant cells, but containing no DNA fragment which can lead in the plant cells to expression of an active transposase.