PROTEIN SEQUENCE-SPECIFIC OLIGONUCLEOTIDE SEQUENCES

摘要

A method for identifying oligomer sequences which specifically bind target proteins or other target moieties is described. The technique involves complexation of the target substance with a mixture of oligonucleotides containing random sequences and sequences which serve as primers for PCR under conditions wherein a complex is formed with some but not all members of the oligonucleotide mixture. The complex is then separated from uncomplexed oligonucleotides and the complexed members of the oligonucleotide mixture are recovered and amplified from the separated complex using the polymerase chain reaction. Successive rounds of selection using complexation, separation, amplification and recovery can be employed. The recovered mixture or oligonucleotide may be sequenced and this information used for the de novo synthesis of the identified oligonucleotide(s). These synthesized oligonucleotides may contain one or more modified bases or backbone linkages.