发明名称 |
TEST TO DETERMINE AN ORGANISM'S SPECIES AND/OR POPULATION IDENTITY BY DIRECT NUCLEOTIDE SEQUENCE ANALYSIS OF DEFINED SEGMENTS OF ITS GENOME |
摘要 |
A method is provided for the determination of the genus, and then the species, and/or strain, and/or subspecies and/or subset of an eukaryote or a prokaryote organism, e.g. a mammal, a bird, a reptile, an amphibian, a fish or an invertebrate. The method includes the first step of isolating DNA from the sample. A defined segment of the DNA is then amplified. The nucleotide sequence of the amplified segment is then determined. That DNA sequence is then compared with a data base of DNA sequences from known species. A cladistic analysis of these sequence data is then carried out. A kit is also provided comprising a carrier; compartments within the carrier to receive one container containing DNA from the above described defined organism; a second container containing an amplified segment of the DNA from that defined organism; and a third container containing DNA sequences for a plurality of genera, and species, and/or strain, and/or subspecies and/or subset or DNA derived therefrom. |
申请公布号 |
WO9205277(A1) |
申请公布日期 |
1992.04.02 |
申请号 |
WO1991CA00345 |
申请日期 |
1991.09.25 |
申请人 |
DAVIDSON, WILLIAM, SCOTT;BARTLETT, SYLVIA, ERNESTINE |
发明人 |
DAVIDSON, WILLIAM, SCOTT;BARTLETT, SYLVIA, ERNESTINE |
分类号 |
C12Q1/00;C12Q1/68 |
主分类号 |
C12Q1/00 |
代理机构 |
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