摘要 |
NEW MATERIAL:Carcinostatic substance TF-1323. It has the following characteristics: appearance, light grey to pale brown powder; melting point, no definite melting point, starts to decompose at 110 deg.C, and vogorously decomposes at >=200 deg.C; elemental analysis, C 29.9-39.4%, H 3.9-5.6%, N 2.8-5.4%; solubility, soluble in water, insoluble in methanol, acetone, chloroform, diethyl ether, etc.; color reaction, positive to Molish reaction, phenol-sulfuric acid reaction, etc., negative to ninhydrin reaction. USE:A carcinostatic agent. The substance inhibits the proliferation of the cells of Ehrlich ascites carcinoma, Ehrlich nodular carcinoma, and sarcoma 180 cancer, and has immono-activating activity. PROCESS:TF-1323-producing bacteria belonging to Fusobacterium genus, e.g. Fusobacterium nucleatum TF-031 (FERM-P No.5077), is cultured, and the clutured liquid is mixed with a hydrophilic organic solvent to obtain the precipitate. The objective TF-1323 is separated from the water-soluble component of the precipitate. |