| 摘要 |
PURPOSE:To enable transduction of a recombinant DNA into plants, etc., by containing a DNA capable of coding a specific amino acid sequence in a Chlamydomonas carbonic anhydrase cDNA. CONSTITUTION:A Chlamydomonas strain is cultured under conditions of ventilation at a high CO2 concentration, and then cultured under conditions of ventilation at a low CO2 concentration to provide cells (A), which are subsequently crushed, precipitated and fractionated with ammonium sulfate to afford a protein (B) having carbonic anhydrase (CA) activity. On the other hand, the ingredient (A) is subjected to treatment such as decomposition, linking and transduction to provide a CAcDNA library (D). A DNA containing the ingredient (C) is then synthesized to prepare a probe (E), which is used to screen the ingredient (D) and produce a Chlamydomonas carbonic anhydrase cDNA composed of the DNA capable of coding an amino acid sequence, expressed by formula III, etc., and containing a DNA expressed by formula IV, etc. |
