| 摘要 |
<p>PURPOSE:To obtain the title hybridoma containing little impurities, with high specificity, capable of secreting in high reproducibility anti-Candida antibody having high potency, by fusion of antibody-productive cells with cells capable of in vitro long-term subculture. CONSTITUTION:An animal species including human being is immunized with Candida, and antibody-productive cells (plasma cells and lymphocytes as their precursors) are prepared from host with its antibody titer to the Candida in the serum sufficiently enhanced. Thence, the antibody-productive cells are fused with myeloma cells in the presence of a fusion promotor, and the resultant hybridoma is separated from the parent cells followed by cloning using limiting dilution method or soft agar technique etc., thus establishing the objective hybridoma (e.g. CD-1-CD-9). The resultant hybridoma is incubated in vitro or in vivo to produce anti-Candida antibody. Use of this antibody will enable rapid and highly reliable immobilization of Candida.</p> |
