N-acetylhexosamine-dehydrogenase, process for producing same, method for the quantitative analysis of N-acetylglucosamine or N-acetylgalactosamine using same and kit for use in the quantitative analysis

摘要

N-Acetylhexosamine-dehydrogenase which takes off hydrogen from N-acetylglucosamine or N-acetylgalactosamine to convert them to N-acetylglucosaminolactone or N-acetylgalactosaminolactone, respectively, and, at the same time, reduces co-enzymes NAD+ to NADH is provided herein. The enzyme of this invention can be obtained by culturing, in a medium, a strain belonging to Genus Pseudomonas and having an ability to produce N-acetylhexosamine-dehydrogenase, followed by collecting the enzyme from the cultured product. Herein is also provided a method for quantitatively analyzing N-acetylglucosamine or N-acetylgalactosamine which comprises reacting N-acetylglucosamine-dehydrogenase upon a sample containing N-acetylglucosamine or N-acetylgalactosamine and measuring the quantity of the resulting NADH.