| 摘要 |
A method of measuring fluorescence emitted by a fluorophore when assaying a substance (analyte) in a sample, wherein a measured florescence signal is compared with a standard curve in which signal strenght (emission) is a function of the analyte concentration. The method is mainly characterized in that (i) the fluorescence values which correspond to the analyte concentration interval (c) of the standard curve are measured at the same emission wavelength (lambdaem) or at the same excitation wavelength (lambdaex) wherein the interval (c) is divided into a finite number of part-intervals C1,.. Ca,... Cn, where a &cir& - is an integer 1 </= a </= n and n &cir& - is an integer >/= 2 and (ii) that the signal (fluorescence)emitted from the sample is measured for the excitation wavelength and at the emission wavelength respectively which correspond to the wavelengths used when measuring corresponding values on the standard curve. |
