发明名称 |
METHOD FOR THE RECOMBINANT PRODUCTION OF HIRUDINS AND HIRUDIN-LIKE POLYPEPTIDES |
摘要 |
2063575 9117250 PCTABS00008 A hirudin or hirudin-like polypeptide having anti-thrombin activity is prepared by recombinant DNA methodologies. The hirudin or hirudin-like polypeptide is preferably expressed in E. coli or insect cells. The hirudin-like polypeptide is preferably a hybrid protein from HV1 and HV2 having the following amino acid sequence: HV12: Val-Val-Tyr-Thr-Asp-Cys-Thr-Glu-Ser-Gly-Gln-Asn-Leu-Cys-Leu-Cys-Glu-Gly-Ser-A_ sn-Val-Cys-Gly-Gln-Gly-Asn-Lys-Cys-Ile-Leu-Gly-Ser-Asp-Gly-Glu-Lys-Asn-Gln-Cys_ -Val-Thr-Gly-Glu-Gly-Thr-Pro-Asn-Pro-Glu-Ser-His-Asn-Asn-Gly-Asp-Phe-Glu-Glu-I_ le-Pro-Glu-Glu-Tyr-Leu-Gln where the underlined sequence is the HV2 portion.
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申请公布号 |
CA2063575(A1) |
申请公布日期 |
1991.11.11 |
申请号 |
CA19912063575 |
申请日期 |
1991.04.05 |
申请人 |
FARMITALIA CARLO ERBA S.R.L. |
发明人 |
BENATTI, LUCA;CARMINATI, PAOLO;LANSEN, JACQUELINE;MAZUE, GUY;RONCUCCI, ROMEO |
分类号 |
A61K38/55;A61K38/00;A61P7/02;C07K14/00;C07K14/815;C12N;C12N1/21;C12N5/10;C12N9/99;C12N15/09;C12N15/15;C12N15/62;C12P21/02;C12R1/19;(IPC1-7):C12N15/15;C12N1/19;A61K38/58 |
主分类号 |
A61K38/55 |
代理机构 |
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